Match Each Label to the Correct Cell It Describes.

For each cell and at each position within the field of view the bright-field images contour coordinates as well as area and circularity are stored during the experiment for later analysis. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often.

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In a post-processing step cell contour coordinates are used to calculate the time traces of the remaining shape descriptors.

. These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community.

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